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How to transfect S2 cells?

How to transfect S2 cells?

During transfection and selection keep cells in the same culture vessel. For general maintenance of cells, pass S2 cells when cell density is between 6 to 20 x 106 cells/ml and split at a 1:2 to 1:5 dilution. Note: S2 cells do not grow well when seeded at a density below 5 x 105 cells/ml.

Why use S2 cells?

One of the most commonly used lines, S2 cells, is particularly useful as it is easy to grow and maintain in the lab, is highly susceptible to gene inhibition using RNAi and is well suited to high-resolution light microscopic assays.

What is S2 cell?

S2 cells are a mathematical mechanism that helps computers translate Earth’s spherical 3D shape into 2D geometry. You can think about them as tiny units of geography that computers understand and developers love to use.

What are Sf9 cells used for?

Sf9 cells, a clonal isolate of Spodoptera frugiperda Sf21 cells (IPLB-Sf21-AE), are commonly used in insect cell culture for recombinant protein production using baculovirus. They were originally established from ovarian tissue. They can be grown in the absence of serum, and can be cultured attached or in suspension.

How does S2 library work?

The S2 library defines a framework for decomposing the unit sphere into a hierarchy of cells. Each cell is a quadrilateral bounded by four geodesics. Each cell in the hierarchy has a level, defined as the number of times the cell has been subdivided (starting with a face cell). Cells levels range from 0 to 30.

What are the two cell types?

There are two main types of cells: prokaryotic cells and eukaryotic cells. Prokaryotic cells include bacteria and archaea. Prokaryotes—organisms composed of a prokaryotic cell—are always single-celled (unicellular). Prokaryotic cells don’t contain a nucleus.

What is a transient transfection?

In transient transfection, the introduced nucleic acid exists in the cell only for a limited period of time and is not integrated into the genome. However, the high copy number of the transfected genetic material leads to high levels of expressed protein within the period that it exists in the cell.

Where do Sf9 cells come from?

The Sf9 insect cell line is a clonal isolate derived from the parental Spodoptera frugiperda cell line IPLB-Sf-21-AE, and it is a suitable host for expression of recombinant proteins from baculovirus expression systems (e.g., Invitrogen’s Bac-to-Bac® and Bac-N-Blue™ Expression Systems).

What is baculovirus expression system?

The Baculovirus Expression Vector System (BEVS) is used to produce recombinant proteins. A baculovirus is an enveloped double-stranded DNA virus that belongs to the Baculoviridae family, which affects approximately 600 different insect species.

What is S2 mapping?

S2 Space-Filling Curve It is constructed by mapping 6 copies of the Hilbert curve to the 6 faces of a unit cube, reflecting and rotating the curves as necessary so that that they link together seamlessly into a continuous loop. The cube is then mapped to the unit sphere using a transformation that minimizes distortion.

Where does the S2 cell line come from?

Introduction The S2 cell line was derived from a primary culture of late stage (20-24 hours old) Drosophila melanogaster embryos (Schneider, 1972). Many features of the S2 cell line suggest that it is derived from a macrophage-like lineage.

What kind of medium is used for S2 cells?

• The complete medium for S2 cells is Schneider™s Drosophila Medium containing 10% heat-inactivated FBS. This medium is used for transient expression and stable selection. Schneider™s Drosophila Medium is available separately from Invitrogen (Catalog no. 11720-034).

Can a Drosophila Schneider 2 cell be transfected?

Transfect S2 cells. Introduction. Drosophila Schneider 2 (S2) cells can be transfected with the recombinant expression vector alone for transient expression studies or in combination with a selection vector (e.g., pCoHygro or pCoBlast) to generate stable cell lines.

When to pass S2 cells in a culture vessel?

During transfection and selection keep cells in the same culture vessel. • For general maintenance of cells, pass S2 cells when cell density is between 6 to 20 x 106 cells/ml and split at a 1:2 to 1:5 dilution.